NBx Platform™
The NBx Platform™ is a proprietary, high-fidelity, microbial system optimized for the production of plasmid DNA.
Time to say goodbye to E. coli?
With faster growth rates and higher plasmid productivities than E. coli strains, the NBx Platform™ addresses common pain points in the growing global demand for plasmid DNA for RNA therapeutics, high throughput gene production and development, vaccines, and cell and gene therapy applications.
Up to 20X Higher Yield
Learn More
Half the Time
Learn More
Higher Quality than E. coli
Learn More
Lower Endotoxin than E. coli
Learn More
Fully compatible with E. coli infrastructure
Learn More
Scalable to Large Scale pDNA Production
Learn MoreOur Clients
Our Early Access Program (EAP) provides you with a deeper look into the data supporting NBx Platform™ technology and quick access to this drop-in compatible system in the form of competent cells and media. The process for participating in our EAP is as follows:
High Throughput Production
Medium to Large Scale Production
Our Partners
Use common DNA transformation methods
Electroporation or chemical
Select with common antibiotic resistance markers
Kanamycin, Ampicilin, Chloramphenicol, Tetracycline, Spectinomycin
Grow plasmids with common replication origins
pUC, pMB1, p15a, RSF1010, RK2
Use our proprietary media formulations
Application specific, chemically defined and rich
Efficient isolation of high-quality plasmid DNA
Use standard alkaline lysis methods
No specialized manufacturing equipment
Use standard USP & DSP equipment
Handle complex sequences with higer success
>10K bp, ITR, toxic constructs
Safer than E. coli
BSL-1 organism with significantly lower endotoxins
The NBx Platform™ strain is considered a safe bacterium, not associated with disease in healthy adult humans and like E. coli, requires Biosafety Level 1 containment.
Yes, inducing cell competence can be achieved by electroporation or chemical methods.
All most common plasmid replication origins can be utilized, including those from pUC, pMB1, p15a, RSF1010, RK2.
All most common plasmid selection markers can be used, including Kanamycin, Ampicillin, Chloramphenicol, Tetracycline, or Spectinomycin.
Yes, the NBx Platform™ uses proprietary, application specific media.
Yes, standard alkaline lysis methods can be used.
No, the strain can be handled and cultivated in standard USP and DSP equipment.